Every year Department of Biotechnology conduct a DBT-JRF BET examination for BIOTECHNOLOGY and BIOINFORMATICS students,if you consider both part A and Part B then there is ~15 to 20 questions which are asked from BIOINFORMATICS,because this year BINC(BINC-2015) is also going to conduct in which BIOINFORMATICS play major role;so for these reasons i have decided to consider all BIOINFORMATICS questions,which was asked in DBT-JRF (2008-2014) Question papers.For that i have prepared "BIOINFORMATICS QUIZ" which not only help you to do good score in DBT-JRF BET-2015 but it is also useful in BINC-2015(part A MCQs).I have selected All these questions from DBT-JRF (2008-2014)Question papers;which are available on DBT Website (http://dbtindia.nic.in/uniquepage.asp?id_pk=16)
Points to Remember :
- Option which is given in BOLD RED colour is a answer of that question.
- If you find out any doubt for particular question or you think that answer which is given is not right; please cross check it with DBT Website (http://dbtindia.nic.in/uniquepage.asp?id_pk=16)or you can inform me on my email: email@example.com.
- please send me complete question including all four options and also consider in which option you have doubt and according to you which option should be right and why????....Thanks .
Bioinformatics Quiz: Test Your Knowledge of Bioinformatics
syllabus of Bioinformatics in DBT-JRF BET examination
1. Major Bioinformatics Resources:
- Sequence databases, Gene Expression database: GEO, SAGE, 3D Structure Database: PDB, NDB, Knowledge driven Databases & utility, Pattern Sequence: InterPro, Prosite, Pfam, ProDom, Gene Ontology
2. Database Searches:
- Keyword-based searches using tools like ENTREZ and SRS Sequence-based searches: BLAST and FASTA
3. Sequence Analysis, Basic concepts:
- Sequence similarity, identity and similarity, definitions of homologues, orthologues, paralogues, Tandem and Interspersed repeats, repeat finding.
4. Scoring Matrix,
- Pairwise sequence alignments, Multiple sequence alignments (MSA), Application in Taxonomy and phylogeny, Comparative genomics.
5. Structural Biology:
- 3-D structure visualization and simulation, Basic concepts in molecular modeling: different types of computer representations of molecules. External coordinates and Internal Coordinates, Molecular Mechanics, Force fields
- Secondary structure elucidation using Peptide bond, phi, psi and chi torsion angles, Ramachandran map, anatomy of proteins – Hierarchical 12organization of protein structure –like CATH,SCOP, FSSP .
7. DNA & RNA secondary and tertiary structures, t-RNA tertiary structure
8. Classification and comparison of protein 3D structures:
- Secondary structure prediction: Algorithms viz. Chou Fasman, GOR methods,
- Tertiary Structure prediction: Fundamentals of the methods for 3D structure prediction (sequence
similarity/identity of target proteins of known structure, fundamental principles of protein folding etc.
- Homology/comparative Modeling, fold recognition, threading approaches, and ab initio structure prediction methods. CASP. Computational design of Promoters, Proteins & Enzymes.
9. Application in drug design:
- Chemical databases like NCI /PUBCHEM. Fundamentals of Receptor-ligand interactions.
- Structure-based drug design: Identification and Analysis of Binding sites and virtual screening.
- Ligand based drug design: Structure Activity Relationship – QSARs & Pharmacophore etc. In silico predictions of drug activity and ADMET.